《植物生理学报》 2018, 54(9): 1507-1515

光质对番茄果实中糖含量和代谢相关酶及基因表达的影响

董飞¹, 王传增², 任煜倩¹, 张现征¹, 王立霞¹, 刘世琦^1,*

¹山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点实验室, 山东泰安271018; ²山东省果树研究所, 山东泰安271000

通信作者：刘世琦；E-mail: liusq99@sdau.edu.cn

摘　要：

以番茄(Solanum lycopersicum)品种‘Micro-Tom’为试材, 待种子发芽后30 d移入光质实验室, 在不同红蓝光处理的番茄果实绿熟期、转色期、成熟期分别取样, 测定可溶性糖含量、糖代谢相关酶活性以及相关基因的相对表达。结果表明, 在番茄果实发育过程中, 红/蓝(3:1)照射处理的果实可溶性糖含量最高, 而且与其他处理之间差异显著。通过对番茄果实中糖含量、糖代谢相关酶和基因相对表达量之间的相关性进行分析可知, 果糖-1,6-二磷酸酶(FBP)、丙酮酸激酶1 (PK1)、丙酮酸激酶2 (PK2)基因表达对葡萄糖、果糖含量的增加起到关键性作用; 中性转化酶(NI)活性和FBP、PK2、NI基因表达对蔗糖、淀粉含量的增加起到决定性作用。

关键词：番茄; 糖代谢; 糖代谢相关酶; 基因表达

修定：2018-09-12

资助：国家公益性行业(农业)科研专项、园艺作物设施栽培光环境精准调控关键技术研究与示范(201303108)。

Effects of light qualities on sugar contents, activities and gene expression of related enzymes involved in sugar metabolism of tomato fruit

DONG Fei^{1, WANG Chuan-Zeng2, REN Yu-Qian1, ZHANG Xian-Zheng1, WANG Li-Xia1, LIU Shi-Qi1,*}

¹College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/Ministry of Agriculture KeyLaboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, Tai’an, Shandong 271018, China; ²Shandong Institute of Pomology, Tai’an, Shandong 271018, China

Corresponding author: LIU Shi-Qi; E-mail: liusq99@sdau.edu.cn

Abstract:

Tomato ‘Micro-Tom’ was used as the test material. After 30 days of seed germination, the seedlings were transferred to the light quality laboratory. The fruits were harvested at the mature green, breaker and ripe stages, and then the fruit soluble sugar content, sugar metabolism related enzyme activity and gene expression were determined. The results showed that the soluble sugar content of red/blue (3:1) was the highest during the development of tomato fruit, and the difference was significant. By analyzing the correlation coefficients of soluble sugar content, sugar metabolism related enzymes and corresponding gene relative expression in tomato fruit, it was found that fructose-1,6-bisphosphatase (FBP), pyruvate kinase 1 (PK1), and pyruvate kinase 2 (PK2) gene expression played a key role in the increase of glucose and fructose. Neutral invertase (NI) activity and FBP, PK2, NI gene expression played a decisive role in the increase of sucrose and starch content.

Key words: tomato; sugar metabolism; glucose metabolism related enzymes; gene expression